The study, published in the journal ACS Sensors, said the team developed a palm-sized test kit, where a person spits into a cartridge and puts it into a processing platform. Within 45 minutes, test results are sent to a custom Android app developed by the researchers.
Principal investigator Weihua Guan, associate professor at Penn, said, “It takes about an hour to develop PCR test results in a lab, but you need to send the sample to a lab and the time it takes in the lab to process it.” Have to keep that in mind.” State University in America.
“We wanted to create a viable alternative to PCR for people to use at home, without having to endure invasive nasal testing,” Guan said.
The platform uses reverse transcription loop-mediated isothermal amplification, or RT-LAMP, for virus detection.
The test device first heats the saliva to 203 degrees Fahrenheit, the temperature at which the viral particle shells break apart and release their genetic material. The genetic material is then mixed with pre-packaged reagents in a microfluidic cartridge.
Finally, the sample is cooled to 149 degrees Fahrenheit, triggering another chemical reaction in which certain viral molecules are multiplied into billions of copies, making the virus easier to identify. If the virus is present in the saliva sample, the user will receive a positive result on their connected smartphone app.
To test the setup, they infected commercially purchased human saliva samples with inactivated SARS-CoV-2 virus particles and ran the samples through the prototype. They also tested some clinical samples. The platform accurately determined whether every sample was positive or negative for the virus.
August 06, 2022 Other New York
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